Safe Daily Intake Range of Cyclocarya paliurus (Cpa) Leaves as a Human Dietary Supplement

Shun Di Tea Industry Co., Ltd. & Green Sugar Technology Information
Published: Tuesday, May 20, 2025

Overview

Based on nutrient content data from four spring Cyclocarya paliurus (Cpa) leaf samples, along with FDA Daily Reference Values (DRV) and Tolerable Upper Intake Levels (UL), this analysis determines the safe adult daily intake range for Cpa leaf powder in tablet or gummy supplement form. The goal is to ensure adequate nutritional benefit without exceeding long-term reference intake limits (i.e., safety thresholds).

1. Sample Identification

SampleDescription
Sample 1Shunhuang Mountain – Black Tea Processing Method (April 2025)
Sample 2Shunhuang Mountain – Green Tea Processing Method (April 2025)
Sample 3Wugang – White Tea Processing Method (April 7, 2025)
Sample 4Wugang – Freeze-Dried Fresh Leaves Processing Method (April 7, 2025)

2. Nutritional Content per 100g of Cpa Dry Leaf (Average of Samples 1–4)

NutrientValue
Total Energy1,212 ± 38 kJ
Protein29.38 ± 3.58 g
Total Fat2.54 ± 0.56 g
Available Carbohydrates19.90 ± 4.01 g
Total Dietary Fiber35.08 ± 3.10 g
Caffeine0 mg (not detected)
Crude Polysaccharides2.89 ± 1.10 g
Total Flavonoids1.61 ± 0.66 g
Total Saponins0.08 ± 0.07 g
Calcium (Ca)921.8 ± 40.5 mg
Chromium (Cr)0.7 ± 0.6 mg
Copper (Cu)2.2 ± 0.1 mg
Iron (Fe)25.5 ± 2.0 mg
Magnesium (Mg)323.0 ± 67.1 mg
Manganese (Mn)223.9 ± 112.4 mg
Potassium (K)2,157.5 ± 287.9 mg
Selenium (Se)0.05 ± 0.06 mg
Vanadium (V)0.04 ± 0.01 mg
Zinc (Zn)9.9 ± 0.9 mg
Molybdenum (Mo)0.02 ± 0.01 mg
Vitamin A0 mg (not detected)
Vitamin E (α-Tocopherol)37.2 ± 7.1 mg

3. Recommended Daily Safe Intake

Recommended: 2g per day. Do not exceed 4g per day.

Rationale

Manganese (limiting nutrient): 2g of Cpa leaf per day provides 6.7 mg of manganese, equivalent to 292% of the DRV, but still below the UL. At 4g per day, the manganese intake reaches 13.4 mg, exceeding the UL and posing a toxicity risk.

Other minerals: At a 2g daily intake, all other minerals (calcium, copper, iron, magnesium, selenium, zinc, molybdenum) contribute between 1% and 5% of their respective DRVs — well below their ULs. Chromium is a moderate exception at approximately 74% of DRV.

Bioactive compounds: At 2g per day, Cpa leaf provides crude polysaccharides (79.6 mg), flavonoids (45.4 mg), and saponins (17.8 mg) — all within the safe range of 100–200 mg.

Vitamins: At 2g per day, Vitamin E content is 0.89 mg (6% DRV), far below the UL of 1,000 mg. Vitamin A was not detected and is not a concern.

Macronutrients: Contributions are negligible (<3% DRV), presenting no risk.

Variability consideration: Using mean + 2 standard deviations ensures that even at the highest observed nutrient concentrations, intake remains within the safe range.

4. Potential Health Benefits at 2g Daily Intake

  • Manganese (292% DRV): Supports bone health, metabolic function, and antioxidant activity.
  • Chromium (74% DRV) & Vitamin E (6% DRV): Assist metabolic regulation and antioxidant defense.
  • Bioactive compounds (crude polysaccharides, flavonoids, saponins): Provide antioxidant and anti-inflammatory effects.

5. Important Precautions

  • Do not consume ≥ 4g per day — manganese toxicity risk applies at this threshold.
  • Individuals with chronic conditions or those taking medications/supplements that affect mineral metabolism should consult a healthcare professional before use.

6. Maximum Safe Intake Determined by Individual Minerals (Based on DRV and UL)

MineralContent per 1g Dry LeafAmount to Reach 100% DRV (g)Amount to Reach UL (g)Risk Assessment
Manganese (Mn)3.9 mg0.592.8⚠️ Very high — primary limiting factor
Zinc (Zn)0.86 mg12.846.5✅ Well below limits at 2–4g intake
Copper (Cu)0.09 mg10111✅ Safe at moderate intake
Selenium (Se)0.001 mg68.75500✅ Present only in trace amounts
Chromium (Cr)0.01 mg3.5100⚠️ May approach recommended intake above 3g
Magnesium (Mg)5.4 mg7865✅ No risk from dietary sources
Iron (Fe)0.69 mg26.165.2✅ Low risk
Calcium (Ca)32.6 mg4076.7✅ Provides meaningful calcium contribution
Potassium (K)26.4 mg178170.5✅ Very difficult to reach the UL
Molybdenum (Mo)0.002 mg301,333✅ Safe at all practical intake levels

7. Macronutrients — Insufficient Contribution to Reach Meaningful DRV Levels

CompoundContent per 1g Dry LeafAmount to Reach 100% DRV (g)Amount to Reach UL (g)DRVUL
Caffeine0 mgN/AN/A400 mg400 mg
Available Carbohydrates0.199 g1,382—275 g—
Total Dietary Fiber0.351 g80—28 g—
Total Fat0.025 g3,068—78 g—
α-Tocopherol (Vitamin E)0.126 mg1197,92715 mg1,000 mg
Vitamin A0 µgN/AN/A900 µg3,000 µg
DRV = Daily Reference Value | UL = Tolerable Upper Intake Level

8. Next Steps

  1. Expand sample size to gather sufficient data for publication in a peer-reviewed journal.
  2. Optimize processing methods to partially reduce the exceptionally high manganese content before large-scale commercialization, thereby lowering application risk.
  3. Test Cpa black tea infusions for nutritional and heavy metal content, including a comparison of pre- and post-brewing Cpa leaf composition.